Genetics

A major role of cytometry in Genetics is associated with image analysis and fluorescence in situ hybridisation (see also Cytogenetics link). Using these technologies, diagnosis of chromosome rearrangements can be achieved using 24 colour painting (SKY, M-FISH) or interphase analysis with locus specific probes and gains and losses of genetic material quantified using Comparative Genomic Hybridisation (CGH). DNA microarray technology is starting to replace the metaphase chromosome for many areas of study so increasing the resolution of diagnosis. Flow cytometry also plays a role, particularly with sorting for the production of chromosome-specific DNA libraries and paints as well as for cell cycle anaysis and ploidy determinations.

References

• Lauzon W, Sanchez Dardon J, Cameron DW, Badley AD. Flow cytometric measurement of telomere length. Cytometry. 2000 Jun 15;42(3):159-64. Review.
• Mascio LN, Yuen BK, Kegelmeyer WP Jr, Matsumoto K, Briner J, Wyrobek AJ. Advances in the automated detection of metaphase chromosomes labeled with fluorescence dyes. Cytometry. 1998 Sep 1;33(1):10-8.
• Carter NP. Cytogenetic analysis by chromosome painting. Cytometry. 1994 Mar 15;18(1):2-10. Review.
• Johnson LA. Sexing mammalian sperm for production of offspring: the state-of-the-art. Anim Reprod Sci. 2000 Jul 2;60-61:93-107. Review.
• el-Naggar AK, Lovell M, Callender DL, Ordonez NG, Killary AM. Concurrent cytogenetic, interphase fluorescence in situ hybridization and DNA flow cytometric analyses of a carcinoma ex-pleomorphic adenoma of parotid gland. Cancer Genet Cytogenet. 1998 Dec;107(2):132-6. Review.
• Asai T, Bates DB, Boye E, Kogoma T. Are minichromosomes valid model systems for DNA replication control? Lessons learned from Escherichia coli. Mol Microbiol. 1998 Aug;29(3):671-5. Review.
• Michels JJ, Duigou F, Marnay J. Flow cytometry in primary breast carcinomas. Prognostic impact of proliferative activity. Breast Cancer Res Treat. 2000 Jul;62(2):117-26.
• Kashiwagi H, Uchida K. Genome-wide profiling of gene amplification and deletion in cancer. Hum Cell. 2000 Sep;13(3):135-41. Review.
• King W, Proffitt J, Morrison L, Piper J, Lane D, Seelig S. The role of fluorescence in situ hybridization technologies in molecular diagnostics and disease management. Mol Diagn. 2000 Dec;5(4):309-19.
• Lichter P, Joos S, Bentz M, Lampel S. Comparative genomic hybridization: uses and limitations. Semin Hematol. 2000 Oct;37(4):348-57. Review.