Michael (Mike) Keeney

Michael Keeney received his Fellowship, Institute of Medical Laboratory Sciences, in Hematology (FIMLS) in Scotland. He also has an Advanced Registered Technologist (ART) diploma in Hematology from the Canadian Society of Medical Laboratory Scientists.

Since 1992, Mike has worked as the Supervisor of technical operations of Hematology/Flow Cytometry at the London Health Sciences Centre in London, Ontario, and as an Associate Scientist with the Lawson Health Research Institute. He holds a certificate in Departmental Management from the Canadian Hospital Association. He is currently Vice-Chairman of the Hematology Committee for QMP-LS, and is also a consultant for the College of American Pathologists, Diagnostic Immunology Resource Committee.

Mike is a reviewer for Cytometry and Clinical Cytometry, and also sits on the editorial board. He is currently on the planning committees for the ISAC and CCS 2006 meetings.

His major research interests are in the area of rare event analysis. In collaboration with Rob Sutherland from Toronto and Jan Gratama from the Netherlands, Mike helped develop a clinical guideline for CD34 enumeration in hematopoietic stem cell transplantation (the ISHAGE guideline), which is now the most commonly used method for assessing mobilization and graft adequacy. More recently, Mike has been working on rare event analysis in solid cancers using both flow and laser scanning cytometry, including detection and characterization of circulating tumor cells and circulating endothelial cells.

Mike brings several skills to the committee. He has worked closely with various groups both in Europe and North America on standardization in many areas of flow cytometry. Mike has also been active in teaching, and is a faculty member of the Annual Clinical Course on Flow Cytometry. Mike has presented lectures throughout the world, including Korea, Hong Kong, Australia and many parts of Europe.

Selected Relevant Publications (of 72 total):

• Sutherland DR, Anderson L, Keeney M, Nayar R, Chin-Yee IH. The ISHAGE Guidelines for CD34+ Cell Determination by Flow Cytometry. J Hematotherapy 1996, 5:213-226
• Keeney M, Chin-Yee IH, Weir K, Popma J, Nayar R, Sutherland DR. Single Platform Flow Cytometric Absolute CD34+ Cell Counts Based On The ISHAGE Guidelines. Ctometry (Communications in Clinical Cytometry) 34:61-70, 1998
• Gratama JW, Kraan J, Keeney M, Sutherland DR, Granger V, Barnett D. Validation of the single-platform ISHAGE method for CD34+ hematopoietic stem and progenitor cell enumeration in an international multicenter study. Cytotherapy (2003) Vol. 5, 55-65
• Allan, A.L., Vantyghem, S.A., Tuck, A.B., Chambers, A.F., Chin-Yee, I., and Keeney, M. (2005). Detection and quantification of circulating tumor cells in mouse models of human breast cancer using immunomagnetic enrichment and multiparameter flow cytometry. Cytometry 2005 May; 65A (1):4-14.

Campaign Statement

My goals and objectives as an ISAC Clinical Counselor will include:

• To facilitate integration of the many disciplines within analytical cytology. ISAC represents members from basic research, clinical, and translational areas. My expertise in working with diverse groups should prove an asset in recruiting new members to the society and encouraging collaboration across disciplines.
• To promote research, development, and applications in analytical cytology. My experience in clinical laboratory practice and development/application of cytometry methods and analysis for biological research will allow me to encourage and promote new research directions related to ISAC, its members, and the field of analytical cytology.
• To disseminate knowledge of analytical cytology. I plan to encourage groups who use cytometry methods extensively but do not submit manuscripts to Cytometry to consider doing so, particularly as critical aspects of their work are often based on the technologies of flow and image cytometry.
• To provide input, information, and advice to the ISAC Council