CURRICULUM VITAE.

NAME:  Huw Stephen KRUGER GRAY.

 PERSONAL DETAILS:‑

Home address: Apartment 44, 370 Chestnut Hill Avenue, Brighton, MA, 02135-7779, U.S.A..

Telephone:  (617)-975-2868 (answering machine when absent).

 (510)-828-1605 (business mobile ‘phone).

Electronic mail address: ZipKG@yahoo.com

 SCIENTIFIC TRAINING & EXPERIENCE:-

· Research, application, development & tuition of Flow Cytometry, with on‑line computer data acquisition, presentation, off-line data transfer, analysis & archiving.

· Bulk cell sterile (& high-speed) flow-sorting, four-way sorting & single cell flow-cloning.

· Flow cytometric techniques: many & varied, especially immuno-phenotyping (surface & cytoplasmic), D.N.A. study (cell-cycle, ploidy, side-population, etc.), apoptosis, et. al. & assorted combinations.

· Establishment, supervision, management & operation of sophisticated Flow Cytometry Core Facilities, including consultation, liaison, scheduling, budgeting & accounting, with direct involvement in many diverse research & clinical projects. 

· Field instrument technical & applications support, including system installations & user training, diagnosis & trouble-shooting, system up-grades, applications advice & consultation, instrument service, support for instrument sales & trials, etc..

· Confocal Laser Scanning Fluorescence Microscopy & 3-D. Imaging.

· Field testing of novel reagents & prototype instrument systems.

· Computing (software & hardware; mainframe & micro‑computers.) & networking.  

· Microbiological techniques, including continuous chemostat culture & fermentation (design, instrumentation, operation).

· Biochemical analyses.

· Lecturing to undergraduate & medical students; Setting, marking & grading term quizzes, final examinations & practical class reports; Demonstrations for U.K. school 'A' level biology teachers' refresher course, undergraduate practical classes & student tutorials.

· Help & supervision of instrument users, project students & technicians.

· Scientific presentations at international congresses, plus other symposia, seminars & meetings, publications in learned journals.

 CAREER HISTORY:‑

Industrial:-

2002-present: Guava Technologies Inc., 25801, Industrial Boulevard, Hayward, CA, 94545, U.S.A..

 Senior Technical Specialist, providing field technical and applications support for the P.C.A. (Personal Cell Analyser), P.C.A.-96 automated screening cytometer and P.C.A.-96-A.F.P. blue laser system. Based in Boston and covering the U.S. North Eastern territory, from New York City up to Canada and west to Buffalo. Guava is a biotechnology company, which develops, manufactures and markets patented, integrated cellular analysis systems, for the life sciences. The company is changing the way in which cell culture monitoring and cell screening assays are performed, by providing compact micro-volume scale systems that make common assays in cell-based analysis accessible directly at the bench. Guava’s products have broad applications to life sciences research, drug discovery and biopharmaceutical production, but also offer potential for use in clinical testing and blood processing, as well as other areas where simple, single cell analysis is important.

1997-2002: BioTransplant Inc., Building 75, Third Avenue, Charlestown Navy Yard, Charlestown, MA, 02129, U.S.A..

 Scientist, to establish & manage a new core Flow Cytometry facility, serving the entire joint company’s requirements for flow cytometric analysis, cell sorting etc.. Instruments included a new custom-built Cytomation three laser (Argon, mixed gas & HeNe) MoFlo high-speed four-way cell sorter & Becton-Dickinson FACScan. Typically, these research instruments are used for cell cycle analysis, study of apoptosis, signalling, for measuring the reaction of monoclonal antibodies with complex mixtures of cells & for sorting in order to separate individual sub-populations from mixtures of cells.

1997: Biogen Inc., 14 Cambridge Centre, Cambridge, MA, 02142, U.S.A..

 Temporary Consultant in Flow Cytometry, to manage the cytometry laboratory equipped with a Becton-Dickinson twin laser FACStar-Plus & two FACScans.

1997: Cytometry Facility, Dana-Faber Cancer Institute, J319A, 44 Binney Street, Boston, MA, 02115, U.S.A..

 Wrote & delivered a series of lectures comprising an “Introduction to Flow Cytometryâ€?, for users of the D.F.C.I. core flow cytometry facility.

University: Postdoctoral:-

1996-1997: Cytometry Laboratories, Veterinary Medicine School, Basic Medical Sciences Department, Purdue University, West Lafayette, Indiana, 47907-1515, U.S.A..

Seven month temporary appointment as post-doctoral Research Assistant, working on various projects involving laser scanning confocal imaging (Bio-Rad MRC 1024 & DVC 250) as an analytical tool, development of DNA assays, image & data analysis, computing & production of the third in a series of Cytometry CD-ROM disks (e.g. co-ordinator & H.T.M.L. code writer for two sections, author of three papers), plus the first Microscopy CD-ROM, as well as being involved extensively in the teaching programmes of the Cytometry Laboratory.

1995‑1996: Pathology Department, University of Texas Southwestern Medical Centre, Dallas, Texas, 75235-9072, U.S.A..

Post-doctoral Research Fellow on a one-year contract to run the core Flow Cytometry Facility, housing a B.‑D. FACStar-Plus three laser (488nm., U..V.. & pumped-dye) cell sorter, with an Automatic Cell Deposition Unit (A.C.D.U.), plus a FACScan cell analyser (also a prototype FACScalibur in another laboratory) for research applications, with Hewlett Packard 340, 310 & Apple Macintosh computers for off-line data analysis. Other instruments also within the facility, which were used exclusively for clinical applications, include a B.-D. FACSort & a Coulter Epics XL, 

1994‑1995: Pathology Department, Tufts University Medical School, Boston, Massachusetts, 02111, U.S.A..

Research Assistant Professor on the Medical School Faculty, with a one-year contract as Chief of Operations of the Pathology Flow Cytometry Facility, housing a B.‑D. FACStar⁺ twin laser cell sorter with an A.C.D.U., plus a B.-D. FACScan analyser & a P.C. for off-line data analysis.

1986‑1994: Immunology Division, Pathology Department, Cambridge University, Tennis Court Road, Cambridge, CB2 1QP, England.

Post-doctoral Research Associate: "Applications of Flow Cytometry". In charge of the core flow cytometry laboratory, housing an Ortho Cytofluorograph 50‑H twin laser cell sorter, plus two B.‑D. FACScan analysers, each with FACSmate robots & four H.-P.340 computers linked with a FACSnet network.

1984‑1986: Various temporary positions, finishing as Records Assistant for the Suffolk Biological Records Centre, Ipswich, England, writing biological database software, using dBase- II on a PC.

University: Postgraduate:-

1978‑1984: Department of Biology, University of Essex, Colchester, Essex, CO4 3SQ, England.

Degree awarded: Ph.D. Microbiology: 1985.

Project title: "The effect of acidity on the physiology of Arthrobacter globiformis", involving continuous chemostat fermentor culture of an important soil bacterium.

University: Undergraduate:-

1975‑1978: School of Biological Sciences, University of East Anglia, Norwich, NR4 7TJ, England.

Degree awarded: B.Sc. (Hons.) Biological Sciences: Class II(1): 1978.

 SUPPLEMENTARY INFORMATION:-

Membership of Learned Societies:-

Chartered Biologist: Member of the Institute of Biology; International Society for Analytical Cytology; Society for General Microbiology; Royal Microscopical Society; British Society for Immunology.

Other qualifications:-

National Flow Cytometry Resource, annual flow cytometry course: Los Alamos National Laboratories, New Mexico, USA: 2001.

References:-

Available upon request.

Publications:-

Available upon request.