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Flow Cytometry Specialist Print E-mail
Written by Administrator   
Jan 21, 2004 at 12:00 AM

Donald E Walker

ewalk@tgi.net

 360.387.8242

Cell: 360.440.0205

 

PROFESSIONAL EXPERIENCE

 

Celltech R&D Inc. , Bothell,Washington

Senior Associate Scientist- Flow Cytometry 12/95-present

Sole operater of the MoFlo HighSpeed , 5 Color, 4way sorter, used to interrogate a large dynamic range (1um-50um) of events for analysis and sorting by bulk or into plates. Support was given in answering experimental questions for Immunologists, Protein chemists, Molecular Biologists and Chemists. Support and maintenance for two walk up Coulter XLs. Processing and multicolor staining of diverse cell types. Training and support for approx. 20 scientists in XL operation and in analysis of all flow data.

 

COULTER CORPORATION, Miami, Florida 4/94-12/94

Regional Diagnostic Specialist

Flow Cytometry Reagent Sales Representative for approximately 400 accounts in a Mid-Atlantic region (five state area). Used instrument experience to identify new instrument sales opportunities in region. Provided team support during equipment demonstrations.

 

BAXTER HEALTHCARE CORPORATION, Round Lake, Illinois 8/88-3/94

Research Associate II

Key involvement with the inception and development of this multiple instrument core facility as a national resource for the company. Performed all 4color sorting and cloning done at this facility with the FACStar Plus dye laser system. Maintained the FACScan, FACSort and trained users in the acquisition and analysis characteristics of all instruments. Focus of studies were in human hematopoesis and transplantation, much of which involved patient samples received from several teaching hospitals in Chicago.

 

IUPUI SCHOOL OF MEDICINE, Indianapolis, Indiana 10/84-1/88

Department of Hematology/Oncology

Flow Cytometry Research Associate

Involved in all aspects of bringing this multiple instrument facility on line and maintaining its daily operation as the core resource for the School of Medicine. Performed all multiparameter sorting and cloning operations with FACS 440 and Epics 753. Focus on human hematopoesis and transplantation. First successful 4 Color work on a commercial instrument to be publically presented. See Page 2: Professional Skills

 

HOWARD HUGHES MEDICAL INSTITUTE, Houston, Texas 2/82-9/84

Immunology

Flow Cytometry Research Associate

Involved in the establishment of this facility’s instrumentation and performed a variety of sample analysis, sorting and cloning with an EPICS V.

 

 

 

EDUCATION

 

University of Redlands, Redlands, California---BA Philosophy; Biology, Chemistry

 

PROFESSIONAL SKILLS

 

Presentations as a Named Participant/Presenter

 

  • Poster: Proliferation Potential and Growth Factor Requirements for Neutrophil Precursors Produced in Cultures of CD34+ Cells. ASH, December 1993.
  • Poster: Flow Cytometry and Immunocytochemistry Evaluation of Human Megakaryocytic Cells from Cultures of Bone Marrow and Peripheral Blood CD34+ Cells. ASH, December 1993.
  • Poster: Droplet Sorting of Large Particles: An Evaluation of Sort Recovery with Jet Diameters up to 400 Microns. International Society for Analytical Cytology. March, 1993.
  • Presentation: Four Color Immunofluorescence Using the Coulter Epics 753. Coulter Users Meeting, San Francisco, California, 1986.

 

Instrumentation/Cellular Preparation

 

  • Thorough knowledge and use of the acquisition, sorting and analysis capabilities of the EPICS V, FACS 440, EPICS 753, FACStar Plus, FACScan, FACSort, XL and MoFlo flow cytometry systems.
  • As needed, cell separation and multicolor staining performed using typical reagents and methods for such work.

 

PROFESSIONAL SOCIETIES

 

International Society for Analytical Cytology

 

 

 

 

 

PUBLICATIONS

 

  1. Lu, Li; Pelus, Louis M.; Broxmeyer, Hal E.; Moore, Malcolm A.S.; Wacheter, Maria; Walker, Donald E.; Platzer, Erich. Enhancement of the proliferation of human marrow erythroid (BFU-E) progenitor cells by prostaglandin E. Requires the participation of OKT8-positive T lymphocytes and is associated with the density expression of major histocompatibility complex class II antigens on BFU-E. Blood, Vol. 68: No. 1, 126-133, 1986.
  2. Lu, Li; Walker, Donald; Broxmeyer, Hal E.; Hoffman, Ronald; Wen Hu; Walker, Edwin. Characterization of adult human marrow hematopoietic progenitors highly enriched by two-color cell sorting with My10 and major histocompatibility class II monoclonal antibodies. J. Immunology, 139: 1823-1829, 1987
  3. Lu, Li; Walker, Donald; Graham, Cathy D.; Waheed, A.; Shadduck, R.K.; Broxmeyer, Hal E. Enhancement of release from MHC-class II antigen positive monocytes of hematopoietic colony stimulating factors CSF-1 and G-CSF by recombinatnt human tumor necrosis factor-alpha; synergism with recombinatnt human tumor necrosis factor-alpha; synergism with recombinant human interferon gamma. Blood, Vol 72; No. 1, 34-41, 1988.
  4. Lu, Li; Srour, Edward F.; Warren, David J.; Walker, Donald E.; Graham, Cathy D.; Walker, Edwin B.; Jansen, Jan; and Broxmeyer, Hal E. Enhancement of release of granulocyte and granulocyte macrophage colony-stimulating factors from phythohemaglutinin-stimulated sorted subsets of human T lymphocytes by recombinant human tumor necrosis factor-alpha synergism with recombinant human IFN-gamma. J. Immunology, 141: 201-207, 1988.
  5. Srour, Edward F.; Walker, Edwin B.; Walker, Donald E.; and Jansen, Jan. Functional and phenotypical studies of the Leu-4 (CD3+), Leu-1(CD5-) T lymphocyte. Clinical Exp. Immunology, Vol. 73; p. 34-39, Feb. 1988.
  6. Bender, J.G.; Van Epps, D.E.; Stewart, G.C.; and Walker, D.E. 13-cis retinoic acid modulates the lineage of mouse bone marrow cells produced in response to interleukin 3. Exp. Hematology, 18:990, 1990.
  7. Bender, J.G.; Unverazgt, K.L.; Walker, D.E.; Lee, W.; Smith, D.H.; Stewart, G.C.; Van Epps, D.E. Identification and comparison of CD34 positive cells and their subpopulations from normal peripheral blood and bone marrow using multicolor flow cytometry. Blood, 77: 2591-96, 1991.
  8. Bender, J.G.; Unverzagt, K.L.; Walker, D.E.; Lee, W.; Lum, L.; Van Epps, D.E.; Williams, S.F.; To, L.B. Characterization of CD34+ cells mobilized to the peripheral blood during the recovery from cyclophosphamide chemotherapy. Int. J. Cell Cloning, 10:23-25, 1992.
  9. Bender, J.G.; Unverzagt, K.L.; Walker, D.E.; Lee, W.; Lum, L.; Van Epps, D.E.; Williams, S.F.; To, L.B. Characterization of CD34+ cells mobilized to the peripheral blood during the recovery from cyclophosphamide chemotherapy. Advances in Bone Marrow Purging and Processing, 1992. Wiley-Liss Inc., pp 575-582.

 

 

 

  1. Bender, J.G.; Williams, S.F.; Unverzagt, K.L.; Walker, D.; Nottelman, D.; Lee, W.; To, L.B.; Van Epps, D.E. Characterization of chemotherapy mobilized peripheral blood progenitor cells for use in autologous stem cell transplantation. Bone Marrow Transplantation, 10:281-285, 1992.
  2. Bender, J.G.; Unverzagt, K.L.; Walker, D.E.; Lee, W.; Smith, S.; Williams, S.; Van Epps, D.E. Phenotypic analysis and characterization of CD34+ cells from normal bone marrow, cord blood, peripheral blood, and mobilized peripheral blood from patients undergoing autologous stem cell transplantation. Clin. Immun. Immunopath. 70:10-18, 1994.
  3. Bender, J.G.; Unverzagt, K.; Walker, D.E. Guidelines for determination of CD34+ cells by flow cytometry: Application to the harvesting and transplantation of peripheral blood stem cells. In: Hematopoietic Stem Cells. The Mulhouse Manual. 1994. Eds: E. Wunder, H. Sovalat, P. Henon, S. Serka. Alpha Med Press, Dayton, OH. Pp. 31-43.
  4. Qiao, Xiaoying, Bender, J.G.; Walker, D.E. Immunocytochemistry and flow cytometry evaluation of human megakaryocytes in fresh samples and cultures of CD34+ cells. Accepted for publication, Cytometry. 9/18/95. 
  5. Garcia-Martinez, Leon F.; Appleby, Mark W.; Staehling-Hampton, Karen; Andrews, Dawn M.; Chen, Yuching; McEuen, Mark; Tang, Phoung; Rhinehart, Rebecca L.; Proll, Sean; Paeper, Bryan; Brunkow, Mary E.; Grandea, Andres, G.; Howard, Edward D.; Walker, Don E.; Charmley, Patrick; Jonas, Mechthild; Shaw, Steven; Latham, John A.; and Fred Ramsdell. A novel mutation in CD83 results in the development of a unique population of CD4+ T Cells. Submitted: J. Immunol. 12/19/03  

 

Technical Assistance

  . 

  1. Clark, L.B., Appleby, M.W., Brunkow, M.E., Wilkinson, J.E., Ziegler, S.F. and F. Ramsdell. 1999. Cellular and Molecular Characterization of the scurfy Mouse Mutant. J. Immunol. 162: 2546-2554.
  2. Brunkow, M.E., Jeffery, E.W., Hjerrild, K.A., Paeper, B., Clark, L.B., Yasayko, S.A., Wilkinson, J.E., Galas, D., Ziegler, S.F. and F. Ramsdell. 2001. Disruption of a new forkhead/winged-helix protein, scurfin, results in the fatal lymphoproliferative disorder of the scurfy mouse. Nat. Genet. 27: 68-73.
  3. Khattri, R., Kasprowicz, D., Cox, T., Mortrud, M., Appleby, M.W., Brunkow, M.E., Ziegler, S.F. and F. Ramsdell. 2001. The amount of scurfin protein determines peripheral T cell number and responsiveness. J. Immunol. 167: 6312-20
  4. Khattri, R., Cox, T., Yasayko, S.A. and F. Ramsdell. 2003. An essential role for Scurfin in CD4+CD25+ T-regulatory cells. Nature Immunol. 4: 337-342
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