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Research Scientist - Molecular Biology / Cell Therapy / Flow Cytometry Print E-mail
Written by Administrator   
Dec 17, 2003 at 12:00 AM

CURRICULUM VITAE

Name Andrei V. Krivtsov

Address: 425 Broadway St. apt. #6, Somerville, MA 02145 (Home)

Address: ViaCell Inc., 26 Landsdowne St. #580, Cambridge, MA 02139 (Work)

Tel: (617) 669 8532 (Cell)

Tel: (617) 225 3904 (Lab)

e-mail:   akrivtsov@viacellinc.com

CAREER OBJECTIVES:

The type of research position I envisage would include the use of molecular biology, flocytometry and biochemical approaches to enable bringing cell therapy to the routine medical practice.

CAREER HISTORY:

Mar 2002 - present

Research Scientist, Cambridge Research Center, ViaCell Inc., Cambridge, MA

Key responsibilities:

· Establishing a mouse model for studying engraftment of cord blood short-term repopulating stem cells 

· Sorting rare populations of hematopoietic stem cells from cord blood

· Origination and research on Mesenchymal Stem Cells / Undifferentiated Somatic Stem Cell from umbilical cord blood

Achievements:

· Enabling NOD/SCID model for evaluation of short-term repopulating stem cells in company’s product - CB001.

· Determined role of endoglin (CD105) in promotion of engraftment of short-term repopulating hematopoietic stem cells. (ref. A1)

· Showed the effect of cell culture conditions on gene expression profile of cord blood Mesenchymal Stem Cells / Undifferentiated Somatic Stem Cell.

Dec 1999- Mar 2002

Dec’99-June’99 Research Fellow. July’99-Mar’02 Senior Research Fellow, Stem Cell Biology Dept., New York Blood Center, Inc., New York City, NY

Key responsibilities:

· Cloning hematopoietic stem cell specific genes

· Studying functions of cloned gene - Jedi

Achievements:

· Cloning full length cDNA for Jedi gene which is specific for HSC (ref. B1, C2)

Jun 1997 -

Jun 1999

Ph.D. program, Lab of Molecular Endocrinology, Cardiology Research Center, Moscow, Russia.

Key responsibilities:

· Investigation of mitogenic and metabolic signaling in Aorta Smooth Muscle Cells.

· Processes of activation GPI-PLC by growth factors

· Characterization of cell responses to a synthetic peptide derived from insulin.

Achievements:

· Identification of PI3-kinase as a mediator for insulin and EGF dependent activation of GPI-PLC (ref.: paper A1, A2 and A3)

May 1995 -

May 1997

Ph.D. training, Department of Molecular Biology, Max-Planck Institute for Biochemistry, Martinsried, Germany.

Key responsibilities:

· Searching for new members of the phosphotyrosine phosphatase family.

· Investigating the role of phosphotyrosine phosphatases in the regulation of mitogenic and metabolic cellular responses to hormones and growth factors.

Achievements:

· Biochemical characterization of two tyrosine phosphatases - SHP-1and SHP-2.

· Investigation of the regulation of SHP-2 and SHP-1 phosphatase activity and their participation in EGFR and insulin receptor signaling (ref.: paper A4, C3)

Feb 1995 -

April 1995

Rotation program, Lab of Molecular Basis of Learning and Behavior, Institute for Normal Physiology, Moscow, Russia.

Key responsibilities:

· Characterization of expression pattern of BHK gene in mouse brain and comparison with that of CSK.

Achievements:

· Identification of cellular distribution of BHK gene in murine brain.

Sept 1993 -

Feb 1995

Student Volunteer and later Diploma Work, Lab of Molecular Basis of Differentiation and Development, Institute for Molecular Biology, Moscow, Russia.

Key responsibilities:

· Cloning new protein tyrosine kinase genes from early stages of hematopoiesis.

· Characterizations of cloned genes.

Achievements

· Cloning and sequencing of mouse tie receptor tyrosine kinase and BHK non-receptor protein tyrosine kinase (ref.: paper A5, C4)

Jan 1992 -

May 1993

Student Volunteer, Group of Engineering Immunology, Cardiology Research Center, Moscow, Russia

Key responsibilities:

· Isolation and purification of antibody and other biological active proteins

· Production of polyclonal antibodies against several proteins.

Achievements:

· Production of polyclonal anti-b2microglobuline and anti-IgG4 monospecific antibodies. Development of ELISA and RIA diagnostic test systems for b2-microglobuline in clinic

TECHNIQUES USED DURING CAREER:

- Flocytometry: Cell sorting and population analysis; MoFlo, FACScan/calibur

- Hematological methods: Colony assays; Cobblestone Area Forming Cell (CAFC) assay; Long-Term Initiating Culture (LTIC) assay

- DNA/RNA manipulation: protein expression in E.coli, transient and stable protein expression in mammalian cells, DNA sequencing, different PCR techniques, in vitro mutagenesis techniques;

- Cell cultivation, Hybridoma technology;

- Immunochemistry: ELISA, RIA, TFIFA, Western blotting, Immunoprecipitation techniques.

- Tissue sections and in situ hybridization.

- Protein isolation and purification (FPLC, classical methods), DNA, RNA, polyA+-RNA, phospholipid isolation and separation.

- Lipid purification and separation (TLC)

- Enzymatic assays: in vitro kinase assays (including MAP kinase activation), measurement of phosphatase activity, determination of PI3 kinase activity, in vitro deglycosylation assays, ecto-5’-nucleotidase assay,

- Cellular response measurements: insulin-dependent glucose uptake and glycogen synthesis, [3H]-Thymidine incorporation and MTT assays, cell transformation assays.

EDUCATION:

1995 -1999

Ph.D. Studenship - Academy of Medical Sciences, Moscow, Russia.

Ph.D. degree awarded on June 3rd 1999

Thesis entitled: Regulation of the insulin signal by tyrosine phosphotases SHP-1

1989 -1995

M.S., State University for Chemical Technology, Moscow, Russia.

Major subject: Biotechnology

Minor subjects: Biochemistry and Molecular Biology

FELLOWSHIPS, GRANTS and AWARDS:

1999

International J. Soros Science Foundation, award for Ph.D. studentship

1998

International J. Soros Science Foundation, award for Ph.D. studentship

1995 – 1997

Max-Planck Society, grant for postgraduate fellowship (DM 44.000).

1995 -1996

DAAD (German Academic Exchange Service), Award for Postgraduate Study.

1994

International J. Soros Science Foundation, Award to an Outstanding Student.

PUBLICATIONS:

A - Published Articles in Refereed Journals:

1. Krivtsov A.V. Menshikov M.Yu., Tkachuk V.A. Regulation of insulin receptor function. Problems in Endocrinology1999 10(45)

2. Krivtsov A.V. and Tkachuk V.A. Ativation of PI3-kinase is required for hydrolysis of GPI. Problems in Endocrinology 1999, 1(45): 44-47.

3. Krivtsov A.V. and Tkachuk V.A. SH2 domain containing phosphotases and hormonal regulation. Questions of Biological, Chemical and Pharmacological Medicine. 1998, 4: 24-28

4. Tenev T., Keilhack H., Tomic S., Stoyanov B., Stein-Gerlach M., Lammers R., Krivtsov A.V., Ullrich A. and Boehmer F.D. Both SH2 domains are involved in interaction of SHP-1 with the epidermal growth factor receptor but cannot confer receptor-directed activity to SHP-1/SHP-2 chimera. J. Biol. Chem. 1997; 272(09): 5966-70

5. Ershler M., Krivtsov A., Krotkova A., Belyavsky A., Visser J.V.M., BHK encodes a novel murine brain and hematopoietic systems expressed nonreceptor tyrosine kinase. Doklady Akademii Nauk, 1994, 339(5): 679-83

B - In Preparation:

1. A. Krivtsov, M Zinovieva, A. Belyavsky, J.V.M Visser, Jedi - a novel protein containing a divergent DSL domain and EGF-like repeats and expressed in early hematopoietic cells. Will be submitted to Stem Cells

C - Presentations:

1. L. Liqin, T. Vaudrain, Y.-J. Jiang, A. Krivstov, J. Visser, M. Kraus, High Doses of Exogenous TGF-1 Induce the Generation of High-Proliferative Potential Stem/Progenitor Cells: Opposite Effect from Its Cell Cycle Inhibition. American Society of Hematology annual meeting, December 6-9 2003; San Diego, CA Blood 2003 Nov16; 102 (11): 819a

 

2. A. Krivtsov, M Zinovieva, A. Belyavsky, J.V.M Visser, Jedi - a novel protein containing a divergent DSL domain and EGF-like repeats and expressed in early hematopoietic cells. International Society for Cell Therapy 2003 annual meeting May 28-31, 2003; Phoenix, AZ

3. A. Krivtsov, A. Kharitonenkov, M. Stein-Gerlach, A. Ullrich, SHP-1 is a negative regulator of the insulin signal. The 9th International conference of the International Society of Differentiation, September 25-30, 1996 Pisa, Italy.

4. Ershler M. A., Samokhvalov I. M., Krotkova A. V., Krivtsov A. V., Belyavsky A. V., and Visser J. W. M., cDNA cloning, genomic structure and alternative splicing of murine BHK-CTK-NTK gene. 24th Annual Meeting of the International Society for Experimental Hematology, Duesseldorf, Germany, August 27-31, 1995. Experimental Hematology (Charlottesville) 1995, 23(8): 749.

 

References available upon request
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