Summary

Professional Experience

1985 - 2003 BC Cancer Agency

 600 West 10 th Ave

 Vancouver, BC.

Canada

V4K3 K8

EXPERIENCE SUMMARY

Experienced, hands-on Section Head manager, with broad-based experience in the clinical immunology with focus on immuno-phenotyping by flowcytometry.

Proven skills in leading an immunology section in the Cancer Clinic, which serves as a reference laboratory for the whole province of British Columbia including managing staff, preparing budget and developing new technology.

Currently looking for a new challenge after years of working in clinical field.

Work Experience

Section Head Immunology Laboratory

Staffing Support

v Provide supervision, direction and support to the Immunology Laboratory Section staff. This included reviewing work assignments and preparing weekly schedules to ensure that maximum efficiency is achieved in order to meet the established TAT of the specimens send in for phenotyping.

v Train or arrange training of new staff and ensure that staff is proficient in all departmental Standard Operating Procedures (SOP).

v Educated staff on all safety procedures and maintained a safe work environment

v Maintain time books including scheduling vacation and leave.

v Arrange casual staff when necessary

v Interview and provide input for selection of new staff

v Arrange and lead bi-weekly staff meeting

Technical Experience

v Over 18 years of Clinical Flow Cytometric experience with a variety of instruments of both BD and Coulter.

v Developed unique 5-colour off-line analysis protocol. Analysis protocol can be used as an off-line tool, is very rapid and cost effective. This system allows for high volume testing utilising only one instrument.

v Maintain all SOPs to department and national standards

v Monitor and ensure quality control levels are maintained on flow cytometer and ZBI nuclear cell counter.

v Provide assistance and suggestions to all levels of staff, from level one technologists to the Medical Director of the section regarding cost effective ways to perform the phenotyping testing of about 2200 specimens per year.

v Maintain a current working knowledge of technology by performing literature searches, Co-ordinate new and ongoing research projects with investigators

Operational Experience

v Work closely with the Business Affairs Leader, in preparing yearly budget, including staffing, equipment and consumable costing.

v Develop phenotype panels when new products become available. In particular for products that will aid in the diagnosis and prognosis in lymphoma and certain leukaemia.

v Responsible for maintaining equipment and trouble shooting any problems. Work closely with service engineer to resolve problems.

v Co-chair the safety meetings for the Dept. of Laboratory Medicine and perform routine safety inspections.

v Interacts with referring physicians regarding interpretation of laboratory results.

v Prepare specimens for labelling from bone marrow’s, to a varity of tissue samples, i.e. lymphnode to skin biopsies.

v Monitor the daily quality control; correct settings like fluorerescense amplification and compensation of the flowcytometer.

v Maintenance of human cell line, which is a control for TdT cytoplasmic test.

v Responsible for ordering all supplies.

v Interaction with referring hospital staff to ensure correct shipping of specimens.

Professional Experience

1982-1985

Research Technologist T3

Division of Immunology,

Department of Pediatrics, UBC.

4480 Oak St.

Vancouver BC 

Department Head : Dr A.J Tingle

Conducted research concerning Rubella Virus Associated Arthritis.

These investigations involved the development, production and characterisation of Hybridoma cell lines against Rubella virus.

The development of an ELISA based test system, which was used to measure the immune response for Rubella virus in human serum.

1978-1982

Research Technologist

Department of Immunology,

University Children’s Hospital

Utrecht, the Netherlands

Department head:

Prof. Dr. R.E. Ballieux

Characterization of regulatory functions of human T cells factors in an in vitro test system. This involved a large array of techniques include protein purification with affinity columns, radioisotope labeling of T-cells factors and a number of human cell culture techniques.

1975 –1978

Research Immunology Technologist

Department of Clinical Immunology of a Regional Hospital;

De Lichtenberg,

Amesfoort, The Netherlands

Department head: Dr.J.A.M. Kerkhaert

Worked in a hospital laboratory environment, performing tasks included antibody level measuring, in vitro testing of human cellular response. Immuno fluorescene based testing on tissue sections for autoimmune antibodies.

Additional Skills

Trained and have years of experience with several flowcytometers, like the BD analyzer, Coulters Epic-C, Elite, Profile, XL, and currently the FC 500 analyzer.

Proficient in Microsoft Office applications, in particular, Excel which is used to generate complex protocols to analyze the large number of data in flow-phenotyping.

Using a third party software; Winlist version 5.0 from Verity House Software created a semi automated analysis system to immunophenotype specimens with hematopoietic disease. Combined with Microsoft Excel, this system results in a very rapid and unique analysis of flowcytometric data. 

A broad based cell culture experience, from production of mouse monoclonal antibodies of hybridoma cell lines, to complex human cell cultures in a variety of research projects.

Production of Rubella virus and development of novice ELISA testing system for anti Rubella human antibodies

Molecular DNA techniques like southern blotting and DNA extraction of human cells.

Teaching

1993 Post graduate lectures in Immunolgy at BCIT

1994 Workshop for the Canadian Society of Laboratory Technologists:

Several in-house lectures and case reports, two workshops for grade 3 and 5 students at the local Elementary school.

Orientation of Medical Residents and other visitors.

Languages

English, Dutch, limited German and French

Professional Memberships

Current registration with the CSMLS registration no: 1004986

References

Dr. Mukesh Chhanabhai

Director of the Immunology section,

The Department of Pathology and Laboratory Medicine,

 BC Cancer Agency, Vancouver Centre

604-877-6098 local 2062

Dr. Randy Gascoyne

Hematopathologist,

The Department of Pathology and Laboratory Medicine,

 BC Cancer Agency, Vancouver Centre

604-877-6098 local 2097

Kathy Chambers

Chief Technologist,

The Department of Pathology and Laboratory Medicine,

 BC Cancer Agency, Vancouver Centre

604-877-6098 local 2083

Dr. Aubrey Tingle

Aubrey J Tingle, MD, PhD, FRCP(C)

President & CEO

Michael Smith Foundation for Health Research

# 200 - 1285 West Broadway

Vancouver, BC, V6H 3X8

Tel: 604-714-2771

Education:

1972 - 1975

General Laboratory Technologist

Education for Medical technologist, General RT with specialization in Microbiology.

1975 - 1977

Advanced Immunology Technologist

Two year evening course in Applied Immunology, Amsterdam. Undergraduate course leading to a degree comparable to B.Sc.

Continued Education:

Attendance of the ISAC meetings, Northwest flowcytometry users meetings.

Training in a number of flowcytometers from benchtop analyzers to cellsorting capable instruments.

Laboratory safety course.

Several in house workshops related to supervisory duties like performance appraisals, discipline, conflict resolution etc.

Publications

UytdeHaag, F.,Heijnen,C.J.,Pot,C.H.,Ballieux,R. E. 1979.Human B cell activation in vitro: Regulation by antigen-specific human suppressor T cells. In: Antibody production in man: In vitro synthesis and clinical implications.Fauci, A.S. , Ballieux, R. E .p141, Academic Press, New York

UytdeHaag, F.,Heijnen,C.J.,Pot,C.H.,Ballieux,R. E. 1979. T-T interaction in the induction of antigen-specific human suppressor T lymphocytes in vitro. J Immunol.123: 646

Heijnen, C.J., UytdeHaag,F., Pot,C.H. , Ballieux, R.E. 1979 Feedback inhibition by human primed T helper cells. Nature 280: 589

Heijnen, C.J., UytdeHaag,F., Pot,C.H. , Ballieux, R.E.. Regulation of an in vitro antibody synthesis in man: role of human inducer T cells. Symposium on new trends in human immunology and cancer immuno-therapy. Serrou, B and Rosenfeld, C.I. (Eds). Elsevier North Holland Publ. Co 1980

UytdeHaag, F.,Heijnen,C.J.,Pot,C.H.,Ballieux,R. E. Regulation of an in vitro antibody synthesis in man: role of human inducer T cells. Symposium on new trends in human immunology and cancer immuno-therapy. Serrou, B and Rosenfeld, C.I. (Eds). Elsevier North Holland Publ. Co 1980

 UytdeHaag, F.,Heijnen,C.J.,Pot,C.H.,Ballieux,R. E Regulation of an in vitro antibody synthesis in man: role of T cell derived antigen specific suppressor factors. In Human Cancer Immunology, volume 2: Characteristics and evaluation in cancer patients. Serrou, B and Rosenfeld, C.I. (Eds). Elsevier North Holland Publ. Co 1981

Heijnen, C.J., UytdeHaag,F., Pot, K.H. and Ballieux, R.E..1980 Antigen-specific human T-cell factors. 1.T helper factor: biological properties. J Immunology.

UytdeHaag, F.,Heijnen,C.J.,Pot,K.H.and Ballieux,R. E 1980 Antigen specific human T-cell factors. 2 T cell suppressor factor. biological properties. J Immunology.

UytdeHaag, F.,Heijnen,C.J.,Pot, C.H.and Ballieux,R. E 1980 Antigen- specific human T cell factors: 5. Influence of adherent cells in the generation of T cell suppressor factors by Tg cells. In: Human B cell function: Activation and immuno regulation. Eds. Fauci, A.S. and Ballieux, R E. Raven Press, New York. 1982

Heijnen, C.J., UytdeHaag,F., Pot, K.H. and Ballieux, R.E..1980. Regulatory mechanisms in the T cell control of the antigen–induced PFC response in man. Antigen specific T cell suppression. In: Human B cell function: Activation and immuno regulation. Eds. Fauci, A.S. and Ballieux, R E. Raven Press, New York. 1982

Heijnen, C.J., Pot, K.H. Kater L.,Kluin-Nelemans H.C.UytdeHaag,F., and Ballieux, R.E.. Functional analysis of the defective T cell regulation of the antigen–specific PFC- response in SLE patients: differentiation of suppressor precursor cells to suppressor effector cells. Clin exp. Immunol 47:359, 1982

Heijnen CJ, Pot K H, Ballieux RE : Characterization of human T suppresso-inducer, -precursor and –effector lymphocytes in the antigen –specific PFC response. Eur J Immunol 1982

Heijen CJ, UtdeHaag F, Pot KH, Bentwich Z , Ballieux RE : Antigen-specific human T cell factors 3. T cell helper and suppressor factors: immunochemical aspects. Annales d’Immunologie.

Tingle AJ, Pot CH, Chantler JK. 1984. Prolonged arthritis, viraemia, hypogammaglobulinaemia, and failed seroconversion following rubella immunisation (letter). Lancet 1:1475-1476.

Tingle AJ, Chantler JK, Pot KH, Paty DW, Ford DK. 1985. Postpartum rubella immunization: association with development of prolonged arthritis, neurological sequelae, and chronic rubella viremia. Journal of Infectious Diseases 152:606-612

Tingle AJ, Pot KH, Yong FP, Puterman ML, Hancock EJ. 1989. Kinetics of isotype-specific humoral immunity in rubella vaccine-associated arthropathy. Clinical Immunology and Immunopathology 53:S99-S106