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| Jan 03, 2003 at 12:00 AM | |
ERIC O’CONNOR
7417 Herschel Ave. Apt. 7 (858)
245-5464 (cell) La Jolla, CA 92037 (858)
784-8396 (work) EDUCATION:
1992-1996 University
of California, Santa Barbara, Goleta, CA B.S. Cell and Developmental Biology EMPLOYMENT: May
2001-Present Flow Cytometry Operator The
Scripps Research Institute Flow Cytometry Core Facility La Jolla, CA May 2000-May
2001 Research Assistant The
Scripps Research Institute/Scripps Clinic Orthopedics Laboratories of Martin Lotz, Ph.D./ Darryl D’alima, M.D. Dept.
of Experimental Medicine/ Joint Mechanics July
1997-June1999 Research Technician 2 The Scripps Research
Institute Laboratory of Joanna Davies, Ph.D. Laboratory of Nora Sarvetnick, Ph.D. Dept. of Immunology Sept.
1996-May1997 Research Technician The Immune Response Corporation Dept.
of Virology Carlsbad,
CA TECHNICAL EXPERTISE: Flow Cytometry: ·Daily
calibration, operation, and maintenance of FACS Diva, FACS Vantage SE, and FACStar Plus TSO by
Becton Dickinson ·Daily
calibration, operation, maintenance and repair of FACScan, FACSCalibur, FACSort analyzers by Becton
Dickinson ·
High-speed and single-cell sorting of up to eight colors, from various cell lines and cell types
including human, mouse, fly, and bacteria ·Multi-color
analysis using CellQuest, FlowJo, and WinMDI software, cell cycle analysis using ModFit software [AS1]·Providing
assistance and training to facility users in cell preparation, instrument operation, and data analysis Molecular: ·Isolation
and purification of DNA and RNA ·PCR
and RT PCR Cellular: ·Sterile
Tissue Culture ·Radioactive
Cell Mediated Immunoassays ·Isolation
and quantitation of lymphocytes from whole human blood ·Protocol
development and optimization/Experimental Design Animal
Work: ·Monitoring
blood glucose levels of mice ·Isolation
of lymphocytes from spleen, lymph nodes and blood of mice Immunochemistry: ·Electrophoretic
and Chromatographic techniques ·ELISAs
including optimization and experimental design ·Western
Blotting Histology: ·Preparation
of paraffin embedded murine tissues ·Microtome
sectioning of paraffin slides ·Cytohistochemistry
including BrdU and insulin staining ·TUNEL
staining and photography on Olympus Bx-50 Microscope PUBLICATIONS: E O’Connor, EM Roberts; JD Davies, Journal
of Immunological Methods. 1999 Oct. 29; 229(1-2):155-60. Amplification of cytokine specific ELISAs
increases the sensitivity of detection to 5-20 picograms per milliliter. JD Davies, R Mueller, S Minson, E O’Connor, T Krahl, N Sarvetnick, Transplantation. 1999 Jun 27; 67(12) 1583-9. Interleukin-4 secretion by the
allograft fails to affect the allograft-specific interleukin-4 response in
vitro. C King, J Davies, R Mueller, MS Lee, T Krahl, B Yeung, E O’Connor, N Sarvetnick, Immunity. 1998 May; 8(5):601-13. TGF-beta1 alters APC preference, polarizing
islet antigen responses toward a Th2 phenotyepe. JD Davies, E O’Connor,
D Hall, T Krahl, J Trotter, N Sarvetnick, Journal
of Immunology 1999 Nov 15; 163(10):5353-7. CD4+ CD45 RB low-density
cells from untreated mice prevent acute allograft rejection. REFERENCES: Professor Nora
Sarvetnick, Ph.D. The Scripps Research
Institute/Dept. of Immunology Imm23 10550 North Torrey Pines Road, La Jolla, CA 92037 noras@scripps.edu Dr. Joanna Davies,
Ph.D. Torrey Pines Institute for
Molecular Studies 3550 General Atomics
Court, San Diego, CA 92121-1122 Professor Martin
Lotz, M.D. The Scripps Research Institute/Div. Of Arthritis Research 10550 North Torrey
Pines Road, La Jolla, CA 92037 Dr. Darryl D’alima,
M.D. Scripps Clinic/Dept. of Orthopedic
Medicine 11025 N. Torrey Pines
Road Suite 140, La Jolla, CA 92037 Alan Saluk, Director. The Scripps Research Institute/Flow
Cytometry Core Facility Imm20 10550 North Torrey Pines Road La Jolla, CA 92037 |
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| Last Updated ( Dec 03, 2004 at 03:05 PM ) |
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