Core Managers Forum
Contributed by Todd Philbrick   
Jan 21, 2010 at 02:13 PM

ISACs Core Managers Task Force will again be holding a Core Managers Forum at CYTO 2010 in Seattle. So that we can best serve the needs of our audience we want to canvass opinion as to topics to be considered. The following are suggestions from the Task Force, please rank in order of interest with 1 being the highest interest. Also indicate if you have any other topic suggestions, suggestions for speakers or if you are prepared to speak or lead a discussion. Please respond to derek.davies@cancer.org.uk by January 31st.

Potential topics:
Funding in times of recession
Core centralization
Efficient management and utilization of cores
Training for Core Managers
Certification in Cytometry
Staff recruitment and retention issues
Effective Communication with Administration
Biosafety issues
Other....

Last Updated ( Jan 21, 2010 at 02:17 PM )
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CALL FOR NOMINATIONS FOR THE 2010 ISAC MEMBERSHIP AWARD
Written by host   
Jan 21, 2010 at 09:38 AM

images/stories/isaclogo_2008.jpg

January 19, 2010

TO:                 ISAC MEMBERSHIP

SUBJECT:     CALL FOR NOMINATIONS FOR THE 2010 ISAC MEMBERSHIP AWARD

FROM:            J. Paul Robinson, Past President and Chair, ISAC Awards Committee

It is once again time to solicit nominations for the ISAC Membership Award (formerly the ISAC Honorary Fellow of the Society Award). Membership Awards are nominated only via ISAC members nominations. Final selection is by the Awards committee.

The ISAC Membership Award recognizes those individuals with exceptional service to the cytometry community, as well as to the Society and those who have achieved peer recognition for advancing the field of cytometry. The award includes a plaque and lifetime membership in ISAC.

Past Membership Award recipients include:

2008:  Hans J. Tanke

2006:   Gunter Valet & Michael Ormerod

2004:   Anne A. Hurley

2002:   Howard Shapiro

2000:   Harry A. Crissman & Harald B. Steen

1998:   Louis A. Kamentsky; Wolfgang Gohde; Leonard A. Herzenberg

1996:   Myron Melamed & Mortimer L. Mendelsohn

1994:   Klaus Goerttler & Marvin A. Van Dilla

1993:   J. Sebastian (Bas) Ploem

To be nominated for this award, an individual must meet the following criteria:

  • Be an ISAC member for a minimum of 10 years
  • Have participated actively in several ISAC Congresses through chairing sessions and presenting papers
  • Contributed in a significant way to the field of cytometry

To make a nomination for this award:

  • Please submit a written explanation of why you think this person should be considered for the ISAC Membership award based upon the criteria outlined above. Please be specific when referring to the nominee's Congress activities as well as achievements both within the Society and the field of cytometry as a whole. A detailed review of the candidate's qualifications in terms of the above criteria should be provided.
  • The nomination should not be discussed with the candidate and should remain in confidence.
  • Only candidates nominated by ISAC members in good standing will be considered by the committee.
  • Multiple individuals may nominate the same person.
  • Please email your submission to ISAC Executive Director, Todd Philbrick at toddp@isac-net.org no later than Friday, February 5, 2010.

 Judging will be by the Awards Committee, which is chaired by me and includes several Past Presidents of ISAC.

 Winner(s) will be notified by March 5th with the award presented on Monday, May 10, 2010 at CYTO 2010: ISAC's XXV International Congress in Seattle, Washington, USA.

 Please take this opportunity to honor your peers and submit a nomination for this prestigious award!

Last Updated ( Jan 21, 2010 at 10:17 AM )
NIH Funding Opportunity for Participation in Cytometry Standards Development Processes
Written by host   
Jan 15, 2010 at 10:03 AM

The National Institute of Biomedical Imaging and Bioengineering (NIBIB) of the US National Institute of Health (NIH) recently announced the availability of funding to support participation in standards development processes (http://grants.nih.gov/grants/guide/notice-files/NOT-EB-09-006.html). Principal investigators with active grants from NIBIB are eligible for administrative supplements to support travel and investigator effort in standards development activities. ISAC has several ongoing standards development activities, including data standards for both flow and image cytometry and fluorescence intensity calibration, and is willing to support applications for these supplements. Interested investigators should contact ISAC, Executive Director Todd Philbrick (toddp@isac-net.org) for more information.

Histochemical Society's 2010 Annual Meeting
Written by host   
Dec 15, 2009 at 01:01 PM

The Histochemical Society's 2010 Annual Meeting, New Trends in Microscopy and Immunohistochemistry is March 21-23, at the Marine Biology Laboratory, in Woods Hole, MA.  The meeting focus will be breakthroughs in new 3D imaging technologies and the tools required to achieve the technologies full potential and advance cell biological and biomedical research.

Scientific sessions will be a mixture of invited presentations and those chosen from submitted abstracts. There is no charge for the first submitted abstract. More detailed information can be found at:

http://www.histochemistry2010.org/index.html

HCS's successful Short Course Immunohistochemistry and Microscopy immediately follows the meeting utilizing the MBL laboratories.  The course includes lectures and hands-on lab sessions and is limited to 30 participants, March 23-26, 2010.  More detailed information can be found at:

http://immunohistochem.com/

You may also download a two page brochure detailing both programs here:

http://bit.ly/3ckTA5

Registration and Abstract submission is now open. We hope to see you in March!

William Stahl
Executive Director
The Histochemical Society

 

CYTOMETRY NEWS
Written by host   
Dec 14, 2009 at 09:41 AM

CYTOMETRY NEWS

By Ben Butkus,  December 07, 2009

NEW YORK (GenomeWeb News) - Life Technologies' Applied Biosystems business today launched a flow cytometer that uses sound waves to control the movement of cells, marking the company's first entry into the flow cytometry instrumentation market.

The instrument, called the Attune Acoustic Focusing Cytometer, is the first to use sound waves to precisely control the movement of cells, and enables enhanced sample throughput, sensitivity, and accuracy for a range of cell biology applications, Life Tech said.

The technology behind the instrument was developed by scientists at New Mexico's Los Alamos National Laboratory, which in 2006 spun out a company called Acoustic Cytometry Systems to commercialize the platform.

Last November, Invitrogen acquired Acoustic Cytometry Systems for an undisclosed amount in a relatively unpublicized deal; and around the same time Invitrogen and Applied Biosystems completed their merger to form Life Tech.

  • Flow Cytometry's Expanding Niche, Genetic Engineering & Biotechnology News, 29, 20, Nov 15, 2009.  See also www.genengnews.com
  • Innovative Applications of Flow Cytometry Win U.S. and European Researchers Free Accuri C6 Flow Cytometer(R) Systems. 

2009 Creativity Awards Program Recipients Plan to Use their Accuri C6 Flow Cytometer(R) Systems for diverse applications to include: studies range from the growth physiology of microbes in ocean systems to measurement of intracellular calcium in mammalian nerve cells.  See news release from PR Newswire:

http://www.prnewswire.com/news-releases/innovative-applications-of-flow-cytometry-win-us-and-european-researchers-free-accuri-c6-flow-cytometerr-systems-78758662.html

 

Last Updated ( Dec 14, 2009 at 09:43 AM )
Call for Nominations
Written by host   
Nov 25, 2009 at 11:02 AM

images/stories/isaclogo_2008.jpg

TO:                 ISAC Membership

FROM:           J. Paul Robinson, ISAC Past President & Chair, ISAC Nominating Committee

SUBJECT:     ISAC Council Nominations

DATE:            November 24, 2009

It is once again time to nominate some of your fellow members to the ISAC Council. The process provides for the Nominating Committee to select a slate of nominees consisting of at least two candidates for each position available. The deadline date for nominations is Thursday, December 31.  Please email your nominations to Todd Philbrick, ISAC Executive Director at toddp@isac-net.org, or fax to him at 301-634-7429.

The ISAC Bylaws allow for two nomination methods:

1.            Nomination for Consideration by the Nominating Committee
An individual's name is submitted for consideration by the Nominating Committee. This individual may be self nominated or nominated by another ISAC member. The individual submitting the nomination MUST have the agreement of the nominee that he/she is willing to run if selected for the slate. Nomination in this manner does NOT guarantee the individual will be placed on the ballot.

2.            Nomination by Petition
This method requires the signatures of eighteen (18) ISAC members (the equivalent of 5% of the number of votes cast in the preceding election).  This nominee must have already been contacted by the petitioners and agreed to run. Nomination in this manner REQUIRES the nominee's name to be included on the ballot.

All nominators and nominees must be ISAC members in good standing - i.e., Full members who are current in their dues.  Emeritus, Honorary and Student members are not eligible to vote or hold office. Please give consideration to geographical issues.  This is important in developing a balanced slate.

Six positions will be filled in the upcoming election:

  • President-Elect
  • Treasurer
  • Councilor, Technical (2)
  • Councilor, Analytical (1)
  • Councilor, Clinical (1)

Information on these positions can be found in the ISAC Bylaws posted at:

http://isac-net.org/index.php?option=com_content&task=view&id=508&Itemid=42

Nominees will be contacted by the ISAC office for materials needed for the ballot. Voting will be conducted electronically. Once the slate has been determined, ISAC members will be notified by email where they may vote.

Although the process does result in some people not being elected, I can assure you that all who agree to be considered for election are "winners" and have distinguished themselves by their willingness to serve ISAC.

If you have any questions regarding the nominations process, please contact ISAC Executive Director, Todd Philbrick at toddp@isac-net.org or 301-634-7454.

Thank you in advance for your participation in this very important process.

 

 

 

Last Updated ( Nov 30, 2009 at 10:10 AM )
Meeting Information
Written by host   
Nov 13, 2009 at 10:00 AM

Leipziger Workshop

We are happy to announce the upcoming 15th Leipziger Workshop, "Cytomics and Stem Cells" April 22-24,2010, details at: http://www.leipziger-workshop.de. Our Website is open now for registration and submission of lecture and poster abstracts.  Abstract submission Deadline is February 28th, 2010.  The Location for the 15th Leipziger Workshop is again the BioCity Leipzig

(Biotechnological Biomedical Center (BBZ)), Deutscher Platz 5, 04103 Leipzig

 

Prof. Dr. Attila Tárnok

Editor-in-Chief "Cytometry Part A"

Universität Leipzig - Herzzentrum

Klinik für Kinderkardiologie

Strümpellstrasse 39

04289 Leipzig

 

--------------------------------

Irish Cytometry Meeting

The full program of the Irish Cytometry Meeting is now available.

Here is the link where one can find the program, information about the meeting, accommodations, travel, entertainment, etc.:

http://irishcytometrysociety.blogspot.com/ The meeting is accredited with 18 credits by the Institute of Biomedical Science.

Dr. Alfonso Blanco Fernández

Flow Cytometry Core Facilities

UCD - Conway Institute of Biomolecular & Biomedical Research

University College Dublin

Belfield, Dublin 4

IRELAND

T: 00353(0)1 716 6836/6947

Last Updated ( Nov 13, 2009 at 10:03 AM )
Update on Cytometry Certification Market Analysis
Written by host   
Oct 16, 2009 at 03:50 PM

Working together, ISAC and CCS, under the sponsorship of the Wallace H. Coulter Foundation, recently completed a survey of its members and other interested parties to assess the level of interest in a program of certification in cytometry.  The response was outstanding-we got nearly 800 responses back, and we'd like to thank everyone who participated.  The breadth of the response was impressive.  Nearly half of the responses came from outside the US, with more than 50 countries represented.  Respondents came from hospitals, universities, industry, and government, and included basic scientists, both translational and clinical researchers, and clinical practitioners.

The main conclusion of this survey, made not by us but by Professional Examination Services, the organization that conducted the survey, is that there is very strong support for a certification program.  Click here to read more.

Last Updated ( Oct 16, 2009 at 03:55 PM )
JOINT MEETING OF ISAC, CCS and ESCCA
Written by host   
Feb 27, 2009 at 01:07 AM

JOINT MEETING OF ISAC, CCS and ESCCA

OCTOBER 15TH 2009 - Jacksonville, Florida

On October 15, 2009 the International Society for Advancement of Cytometry (ISAC), Clinical Cytometry Society (CCS), and the European Society for Clinical Cell Analysis (ESCCA) held the first ever joint meeting of the Societies.  The one day meeting appealed to basic and translational scientists in addition to physicians and medical technologists working in the field of flow or image cytometry; click here to view the final program.  Attendees were treated to fantastic presentations and workshops from: 

  • Gabriela Dontu, Ph.D., King's College London - Click here to view the presentation
  • Sara Weis, Ph.D., University of California San Diego
  • Mike Andreeff, M.D., Ph.D., The University of Texas
  • Bill Matsui, M.D., Johns Hopkins University, Baltimore, MD
  • Brent Wood, M.D., Ph.D., University of Washington - Click here to view the presentation
  • Jim Jacobberger, Ph.D., Case Western Reserve University, Pittsburgh, PA
  • David Hedley, M.D., University Health Network, Toronto, Canada
  • Wolfgang Kern, M.D., Ph.D., MLL Münchner Leukämielabor GmbH, Munich, Germany
  • Zbigniew Darzynkiewicz, M.D., Ph.D., Brander Cancer Research Institute - Click here to view the presentation

We hope this and future meetings will increase communications within our various societies and it is the genesis

for future collaborations.

 

Last Updated ( Nov 06, 2009 at 10:44 AM )
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Call for CYTO 2011 Congress Chair Applications
Written by host   
Oct 05, 2009 at 09:57 AM

In 2010, ISAC will be moving to annual (from biennial) meetings of the Society.   ISAC Council has voted to create the position of CYTO Congress Chair to relieve the President-Elect of the responsibility of planning two CYTO Congresses.  This volunteer position will report to the ISAC Council, and will be responsible for coordinating the ISAC CYTO Congress in conjunction with the ISAC Council, Executive Director, Organizing Committees, Program Committee and Meetings Management firm.

The ISAC Council is now accepting applications for CYTO 2011 Congress Chair.  The CYTO 2011 Congress will be held from May 20-25 in Baltimore, Maryland, USA.  The application deadline is October 30.  Please click here for more information

Last Updated ( Oct 05, 2009 at 09:58 AM )
ISAC Constitution and Bylaws
Written by host   
Sep 03, 2009 at 12:00 AM

The proposed amendments to ISAC's Constitution and Bylaws have been ratified by a vote of membership.  These amendments have updated the Society's name, logo, changed the length of term for Officers, Councilor, Committee members and revised other outdated information.

The revised Constitution and Bylaws can be viewed by clicking here

Last Updated ( Sep 26, 2009 at 10:59 AM )
FCS 3.1
Written by host   
Sep 24, 2009 at 03:10 PM

The flow cytometry data file standard provides the specifications needed to completely describe flow cytometry data sets within the confines of the file containing the experimental data. In 1984, the first Flow Cytometry Standard format for data files was adopted as FCS 1.0. This standard was modified in 1990 as FCS 2.0 and again in 1997 as FCS 3.0. We report here on the next generation Flow Cytometry Standard data file format. FCS 3.1 is a minor revision based on suggested improvements from the community. The unchanged goal of the Standard is to provide a uniform file format that allows files created by one type of acquisition hardware and software to be analyzed by any other type.
The FCS 3.1 standard retains the basic FCS file structure and most features of previous versions of the standard. Changes included in FCS 3.1 address potential ambiguities in the previous versions and provide a more robust standard. The major changes include simplified support for international characters and improved support for storing compensation. The major additions are support for preferred display scale, a standardized way of capturing the sample volume, information about originality of the data file, and support for plate and well identification in high throughput, plate based experiments. Please see the normative version of the FCS 3.1 specification for a complete list of changes.

Attachments:

Last Updated ( Sep 25, 2009 at 11:41 AM )
Call for Volunteers
Written by host   
Sep 24, 2009 at 03:17 PM

ISAC in need of focus group volunteers to participate in a usability study for a public flow cytometry data repository.  The data repository will store annotated experiments according to the MIFlowCyt standard.  Volunteers will be asked to participate in one or two webex sessions lasting no longer than two hours.  If you are interested please email mhassel@bccrc.ca with your contact information and area of expertise.

Last Updated ( Oct 04, 2009 at 05:16 PM )
New England Cytometry Users Group Fall 2009 Meeting
Written by host   
Sep 23, 2009 at 03:38 PM

The New England Cytometry Users Group would like to announce the date and program for our annual Fall meeting.  It will be on October 22nd from 9 am to 5 pm at the beautiful Starr Center at the Schepens Eye

Research Institute in Boston.  Registration will open at 8:15 am with breakfast being served.

See:  http://www.schepens.harvard.edu/starr_center

We have an excellent line up of speakers this year!

Kamran Badizadegan, MD.
Harvard Medical School / MGH
http://hst.mit.edu/public/people/faculty/facultyBiosketch.jsp?key=Badizadegan

Al Donnenberg, Ph.D.
University of Pittsburgh
http://www.mirm.pitt.edu/people/bios/DonnenbergA1.asp

Joonsoo Kang, Ph.D.
UMASS Medical School
http://www.umassmed.edu/ivp/faculty/kang.cfm

Peter Lopez, AAS
New York University
http://www.med.nyu.edu/research/lopezp04.html

Phil McCoy, Ph.D.
NIH
http://www.nhlbi.nih.gov/resources/chi/directors/mccoy.htm

Jonathan Sexton, Ph.D.
North Carolina Central University
http://brite.nccu.edu/Dr_Sexton

Howard Shapiro, MD., PC
http://www.shapirolab.com/

Frank Traganos, Ph.D.
New York Medical College
http://www.nymc.edu/Research/CoreFacilitiesAndResources/FlowCytometry.html

Please check our web site for meeting updates http://www.bostoncytometry.org 

To register, send a check payable to THE NEW ENGLAND USERS GROUP FOR CYTOMETRY in the amount of $65.00 per attendee to my attention at the address below.  Registration is due no later than 15 October, 2009.

This promises to be a great NECyto meeting with a terrific line up of speakers at an excellent venue.

Best regards and looking forward to see you on the 22nd of October!

Richard F. Konz, Jr.
President; New England Cytometry Users Group
http://www.bostoncytometry.org
Research Assistant Professor
Department of Medicine
Graduate Faculty, Immunology and Virology Program
Director, Flow Cytometry Core Facility
UMass Medical School
55 Lake Avenue North
Room: S5-322
Worcester, MA 01655
Office:  508-856-1598
Lab:     508-856-3276
Fax:   508-856-1581
Pager:  508-722-0560
Email:  Richard.Konz@umassmed.edu
Lab Web:      http://www.umassmed.edu/facslab
Faculty Web: http://www.umassmed.edu/ivp/faculty/konz.cfm

Last Updated ( Sep 25, 2009 at 11:41 AM )
3rd Annual Torrey Pines Optical Technology Symposium
Written by host   
Sep 23, 2009 at 03:36 PM

I am pleased to announce the 3rd Annual Torrey Pines Optical Technology Symposium to be held at The Scripps Research Institute in La Jolla, CA on Friday, November 6th, 2009.  Southern California Core Managers' Meeting will be held the previous evening.

 

We once again have an outstanding line-up of speakers this year:

 

Scott Tanner (University of Toronto, Toronto, Canada): "A Mass Cytometer and Element Tagging System for Massively Multi-parameter Cytometry"

Howard Petrie (Scripps Florida, Jupiter, FL): "Spatial mapping of Thymic Stromal Micro-environments reveals unique features influencing T lymphoid differentiation"

John A. Nolan (La Jolla Biotechnology Institute, La Jolla, CA): "Raman Flow Cytometry: Cell measurements beyond 20 parameters"

Tad George (Amnis, Seattle, WA): "ImageStream X Cytometry: High-speed high-content image analysis of cells in Flow"

Michael W. Olszowy (Invitrogen, Eugene OR): "The Newest and Brightest Molecular Probes Flow Cytometry Reagents"

John Foster (IMT, Santa Barbara, CA): "NanoSorter Rare Cell Purification system "

 

There will be no cost for attending and registration information will be announced shortly.  For more details please see:  http://facs.scripps.edu/TPOT09.html.  For Core Managers' Meeting please see: http://facs.scripps.edu/managers09.html

 

If you have any questions, please feel free to contact me Alan Saluk by email (asaluk@scripps.edu)or phone 858 784 8251

 

2010 NIH Director's Pioneer and New Innovator Award Call for Applications
Written by host   
Sep 08, 2009 at 12:12 PM

Each summer, the National Institutes of Health (NIH) makes grants to exceptionally creative scientists through the NIH Director's Pioneer Award and NIH Director's New Innovator Award programs. The application period for the 2010 Pioneer Award opens on September 20, 2009 and closes on October 20, 2009. The 2010 New Innovator Award application period begins on September 27, 2009 and ends on October 27, 2009. 

For more information please visit http://nihroadmap.nih.gov/pioneer/.

Last Updated ( Sep 08, 2009 at 12:52 PM )
AIMBE’s Council of Societies 4th Annual Federal Symposium
Written by host   
Aug 31, 2009 at 04:28 PM

ISAC is proud to co-sponsor the American Institute for Medical and Biological Engineering's (AIMBE) Council of Societies 4th Annual Federal Symposium on September 14-15, 2009 in Washington, DC.  The theme for this year's Symposium is "Medical and Biological Engineering and the Promise of Healthier Tomorrow."  ISAC members will be represented by Tim Bushnell, Councilor and Todd Philbrick, Executive Director.  For more information the Federal Symposium please click here.

Last Updated ( Sep 01, 2009 at 09:37 AM )
CYTO 2010
Written by host   
Aug 27, 2009 at 10:27 AM

Dear ISAC Member,

CYTO 2010 the XXV International Congress of the International Society for Advancement of Cytometry (ISAC) will be held from May 8-12, 2010 in beautiful Seattle, Washington, USA.

The Organizing Committee calls upon all interested scientists to submit high quality papers for oral presentation at the Congress and publication in the Proceedings issue of CYTOMETRY Part A.  CYTOMETRY Part A is the official journal of ISAC and is widely available in both print and electronic forms.  It is indexed by PUBMED and ISI Web of Knowledge.

Accepted papers will be scheduled for oral presentation at the Congress, and the corresponding author will a special gift from ISAC!

What to do:

1. Submit a manuscript (previously unpublished original research or review paper) to Cytometry Part A through the journal website by the 25th of September.  Be sure to indicate in the cover letter that you are submitting in response to this call for Congress papers.

2. Submitted papers will be reviewed on a fast track, and the decision letter will be sent by October 9.  Papers accepted without revision or accepted with minor revisions will be invited for oral presentation at the congress.  (Papers needing major revisions will not be considered for oral presentation or inclusion in the Proceedings issue, but may be processed as regular publications if desired.)

3. If accepted, the final revised version must be submitted within two weeks of receiving the decision letter.

4. The accepted papers will be published in the APRIL 2010 issue of Cytometry Part A.

Important dates:

Deadline for submission                        September 25, 2009
Notification of acceptance/rejection        October 23, 2009
Submission of revised paper                  November 07, 2009
Deadline for Congress early registration  March 8, 2010

Congress web site:                              Opens at www.isac-net.org on May 15.

Cytometry Part A web site:                   http://www3.interscience.wiley.com/cgi-bin/jhome/33945

Sincerely,

CYTO 2010 Organizing Committee

Last Updated ( Aug 27, 2009 at 11:51 AM )
ERF Study Task Force
Written by host   
Sep 02, 2009 at 12:00 AM

Call for Participants:

ISAC Task Force on Fluorescence Calibration

 The ISAC Standards Committee is commissioning a Task Force to evaluate a new approach to fluorescence intensity calibration in flow cytometry. We invite participation from instrument and reagent manufacturers, as well as from users interested in fluorescence intensity calibration. Interested persons should send their contact info and any other relevant information to ISAC Executive Directors Todd Philbrick (toddp@isac-net.org).

Summary

For the calibration of fluorescence measurements, and flow cytometry measurements especially, the use of the Mean Equivalent Soluble Fluorophoes (MESF) as an intensity measurement standard has facilitated the reporting of absolute intensity measurements. Reference particles whose intensities are calibrated in MESF units are available for a few of the most frequently used fluorophores (for example, fluorescein and phycoerythrin), but extension of this approach to highly multiparameter measurements is limited by the availability of reference materials.

An alternative approach is to use a calibration particle with a broad emission spectrum and referenced to a single reference fluorophore to calibrate the response of multiple fluorescence channels over defined spectral ranges. We refer to this as the Equivalent Reference Fluorophore (ERF) unit. The ERF unit has practical advantages with respect to the MESF unit, most notably the possibility to use a single reference particle for calibration across multiple fluorescence channels. The ERF unit will complement the MESF unit by providing practical and usefully accurate calibration of absolute intensities for many fluorescence channels.

Calibration in ERF units is not restricted to multifluorophore beads.  Fluorochrome specific beads may also be calibrated in ERF intensity units to reference ERF standards.  Cross calibration of fluorochrome-specific beads (e.g. stained with fluorescein or phycoerythrin) to multifluorophore beads will be practical using ERF units.

Reference

L Wang, AK Gaigalas, G Marti, F Abbasi, and RA Hoffman.  Toward Quantitative Fluorescence Measurements with Multicolored Flow Cytometry.  Cytometry 73A: 279-288 (2008).

 

Last Updated ( Sep 03, 2009 at 01:04 PM )
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